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Rapid Testing of Resistance of Timber to Biodegradation by Marine Wood-Boring Crustaceans
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JoVE Journal Environment
Rapid Testing of Resistance of Timber to Biodegradation by Marine Wood-Boring Crustaceans

Rapid Testing of Resistance of Timber to Biodegradation by Marine Wood-Boring Crustaceans

DOI:
10.3791/62776-v

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14:06 min

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January 29, 2022

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, , , , ,
1University of Portsmouth, 2Microbiology Department,University of Massachusetts

Chapters

  • 00:12Introduction
  • 04:24Preparing the Test Sticks
  • 04:44Vaccuum Impregnation
  • 06:08Leaching Wood
  • 06:38Extracting Gribbles
  • 07:05Identifying Limnoria quadripunctata
  • 07:37Preparing Well Plates
  • 08:02Collecting and Counting Faecal Pellets and Assessing Vitality
  • 09:05Image Processing
  • 09:34Process to Generate Faecal Pellet Count Using Image J
  • 11:43Data Analysis
  • 11:57Representative Results
  • 12:52Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a method for assessing the feeding rate of the wood-boring crustacean, Limnoria, by measuring faecal pellet production. This method is designed for use in non-specialist labs and has potential for incorporation into standard testing protocols, to evaluate enhanced wood durability under marine conditions.

Tags

Keywords: TimberWoodMarineBiodegradationWood-boring CrustaceansShip WormsGribbleBiocidesWood ProtectionRapid TestingTimber Resistance

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