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JoVE Journal Developmental Biology
Analyse af Transforming Growth Factor ß Familie kavalergang produkter udskilles i Blastocoele af Xenopus laevis embryoner
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Analyse af Transforming Growth Factor ß Familie kavalergang produkter udskilles i Blastocoele af Xenopus laevis embryoner

Article DOI: 10.3791/62782-v • 06:57 min • July 21st, 2021
July 21st, 2021 •
1, 2
1Department of Neurobiology, University of Utah, 2Departments of Neurobiology and Department of Internal Medicine, Division of Hematology and Hematologic Malignancies, University of Utah, School of Medicine

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Når Transforming Growth Factor ß familie prækursor proteiner er ektopisk udtrykt i Xenopus laevis embryoner, de dimerize, få kløvet og udskilles i blastocoele, som begynder ved den sene blastula til tidlig gastrula fase. Vi beskriver en metode til at aspirere kavalergangsprodukter fra blastocoele hulrum til immunblod analyse.

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Udviklingsbiologi udgave 173
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