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JoVE Journal Developmental Biology
Analys av transformerande tillväxtfaktor ß Familjeklyvningsprodukter utsöndras i Blastocoele av Xenopus laevis embryon
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Analys av transformerande tillväxtfaktor ß Familjeklyvningsprodukter utsöndras i Blastocoele av Xenopus laevis embryon

Article DOI: 10.3791/62782-v • 06:57 min • July 21st, 2021
July 21st, 2021 •
1, 2
1Department of Neurobiology, University of Utah, 2Departments of Neurobiology and Department of Internal Medicine, Division of Hematology and Hematologic Malignancies, University of Utah, School of Medicine

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När Transforming Growth Factor ß familjeprekursorproteiner uttrycks extopiskt i Xenopus laevis embryon, de dimerize, blir klyvda och utsöndras i blastocoele, som börjar i slutet av blastula till tidigt gastrula stadium. Vi beskriver en metod för aspirerande klyvning produkter från blastocoele hålighet för immunoblot analys.

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Utvecklingsbiologi nummer 173
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