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Journal
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Biology
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マウス心臓全体のメゾスコピック光学イメージング
JoVE Journal
Biology
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JoVE Journal
Biology
Mesoscopic Optical Imaging of Whole Mouse Heart
Please note that all translations are automatically generated.
Click here for the English version.
マウス心臓全体のメゾスコピック光学イメージング
DOI:
10.3791/62795-v
•
08:53 min
•
October 14, 2021
•
Francesco Giardini*
1
,
Erica Lazzeri*
1
,
Camilla Olianti*
1
,
Giada Beconi
,
Irene Costantini
2
,
Ludovico Silvestri
3,4
,
Elisabetta Cerbai
5
,
Francesco S. Pavone
3,4
,
Leonardo Sacconi
3,6
1
European Laboratory for Non-Linear Spectroscopy
,
2
Department of Biology
,
University of Florence
,
3
National Institute of Optics, National Research Council
,
4
Department of Physics and Astronomy
,
University of Florence
,
5
Department of Neurosciences, Psychology, Drugs and Child Health
,
University of Florence
,
6
Institute for Experimental Cardiovascular Medicine, Faculty of Medicine
,
University of Freiburg
Chapters
00:04
Introduction
00:40
Heart Clearing
02:48
Cellular Membrane Staining
04:18
Mounting and Acquisition
06:48
Results: Mesoscopic Optical Imaging of Whole Mouse Heart
08:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
我々は、組織形質転換と染色の新たな進歩と軸走査型ライトシート顕微鏡の開発を組み合わせることにより、マウス心臓全体のメゾスコピック再構成法を報告する。
Tags
Mesoscopic Optical Imaging
Whole Mouse Heart
Clearing Protocol
Mesos PIM Technology
Micrometric Resolution
Mouse Heart
Hydrogel Solution
Clearing Solution
Wheat Germ Agglutinin Alexa Fluor 633
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