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Generation of Naïve Blastoderm Explants from Zebrafish Embryos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

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07:21 min

July 30, 2021

DOI:

07:21 min
July 30, 2021

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Summary

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Zebrafish blastoderm explants are generated by isolating embryonic cells from endogenous signaling centers within the early embryo, producing relatively naïve cell clusters easily manipulated and cultured ex vivo. This article provides instructions for making such explants and demonstrates their utility by interrogating roles for Nodal signaling during gastrulation.

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