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May 20, 2022
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This protocol presents a technique for high-resolution mapping of replication sites in structurally preserved chromatin in situ that employs a combination of pre-embedding EdU-streptavidin-Nanogold labeling and ChromEMT.
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Cite this Article
Sosnovskaya, S., Zakirov, A. N., Ryumina, E. D., Kharybina, E., Golyshev, S. A., Strelkova, O. S., Zhironkina, O. A., Moiseenko, A., Orekhov, A., Kireev, I. I. Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography. J. Vis. Exp. (183), e62803, doi:10.3791/62803 (2022).
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