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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
JoVE Journal
Biology
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JoVE Journal Biology
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

3,691 Views

14:56 min

May 20, 2022

DOI:

14:56 min
May 20, 2022

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Summary

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This protocol presents a technique for high-resolution mapping of replication sites in structurally preserved chromatin in situ that employs a combination of pre-embedding EdU-streptavidin-Nanogold labeling and ChromEMT.

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