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JoVE Journal Cancer Research
In vivo Immunogenicitetsscreening af tumorafledte ekstracellulære vesikler ved flowcytometri af milt-T-celler
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In vivo Immunogenicitetsscreening af tumorafledte ekstracellulære vesikler ved flowcytometri af milt-T-celler

Article DOI: 10.3791/62811-v • 08:02 min • September 23rd, 2021
September 23rd, 2021 •
1,2, 1,2,3,4, 1,2
1Department of Medicine III, School of Medicine, Technical University of Munich, 2Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, 3Department of Internal Medicine III, University Hospital Regensburg, 4National Centre for Tumor Diseases WERA

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Dette manuskript beskriver, hvordan man vurderer in vivo-immunogenicitet af tumorcelleafledte ekstracellulære vesikler (IV'er) ved hjælp af flowcytometri. ELBILER afledt af tumorer, der gennemgår behandlingsinduceret immunogen celledød, synes særligt relevante i tumorimmunovervågning. Denne protokol eksemplificerer vurderingen af oxaliplatin-inducerede immunstimulerende tumor-ELBILER, men kan tilpasses forskellige indstillinger.

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