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In Vivo Immunogenicitet Screening av tumör-härledda extracellulära vesicles av flöde cytometri av splenic T-celler
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
DOI:

08:02 min

September 23, 2021

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Chapters

  • 00:05Introduction
  • 00:48Generation and Isolation of Extracellular Vesicles (EVs) Derived from Tumor Cells After Chemotherapy Exposure
  • 02:59Immunization of C57BL/6 Mice with Extracellular Vesicles (EVs) and Flow Cytometry Analysis of Splenic T Cells
  • 06:01Results: The Assessment of the Immunogenicity of Tumor-Derived Extracellular Vesicles (EVs)
  • 07:21Conclusion

Summary

Automatic Translation

Detta manuskript beskriver hur man bedömer in vivo immunogenicity av tumör cell-härledda extracellulära blåsor (EVs) med hjälp av flöde cytometri. EVs härrör från tumörer som genomgår behandling-inducerad immunogenic cell död verkar särskilt relevant i tumör immunosurveillance. Detta protokoll exemplifierar bedömningen av oxaliplatin-inducerad immunostimulatory tumör EVs men kan anpassas till olika inställningar.

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