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Immunology and Infection

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JoVE Journal Immunology and Infection
Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
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Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

Article DOI: 10.3791/62857-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2, 2,3,4
1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, 3Department of Infectious Diseases, Virology, Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)

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Summary
Automatic Translation

This protocol describes how to infect human intestinal organoids from either their apical or basolateral side to characterize host/pathogen interactions at the single-cell level using single-cell RNA sequencing (scRNAseq) technology.

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Gastrointestinal Organoids Pathogen Infection Single Cell Sequencing Biosafety Level 3 (BSL-3) Conditions Viral Infection Response Seeding Method Single Cell RNA Sequencing Biosafety Regulations Category 3 Pathogens Organoid Cultures Infected Organoids Gel Beads RT Reagent Reducing Agent B RT Enzyme C Template Switch Oligo 3D Organoids
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