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From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow
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From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow

Article DOI: 10.3791/62866-v • 06:08 min • July 22nd, 2021
July 22nd, 2021 •
1,2, 1,2, 1,2, 1,2
1Department of Chemistry and Biochemistry, California Polytechnic State University San Luis Obispo, 2Center for Application in Biotechnology, California Polytechnic State University San Luis Obispo

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Summary
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This work describes the preparation of cell extract from Escherichia coli (E. coli) followed by cell-free protein synthesis (CFPS) reactions in under 24 hours. Explanation of the cell-free autoinduction (CFAI) protocol details improvements made to reduce researcher oversight and increase quantities of cell extract obtained.

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Cell-free Protein Synthesis Autoinduction Workflow In Vitro Protein Synthesis Cell-free Expression Energy Metabolism Cell-free Reactions Autoinduction Media Sugar Solution Inoculate Media E.coli BL21 Star Plate Incubator Centrifuge
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