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JoVE Journal
Genetics

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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
 

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Article DOI: 10.3791/62872-v 12:08 min August 20th, 2021
August 20th, 2021

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Summary

This protocol details a comprehensive approach for the culturing, sequencing, and de novo hybrid genome assembly of urinary bacteria. It provides a reproducible procedure for the generation of complete, circular genome sequences useful in studying both chromosomal and extrachromosomal genetic elements contributing to urinary colonization, pathogenesis, and antimicrobial resistance dissemination.

Tags

Hybrid De Novo Genome Assembly Complete Genomes Urinary Bacteria Short-read Sequencing Long-read Sequencing Genome Architecture Mobile Genetic Elements Culturable Bacteria Double-stranded DNA Pool Paramagnetic Beads Wash Pellet Ethanol Washes Nuclease-free Water
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