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Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering
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JoVE Journal Engineering
Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering

Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering

DOI:
10.3791/62873-v

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07:53 min

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August 06, 2021

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, , , ,
1NIST Center for Neutron Research,National Institute of Standards and Technology, 2Department of Chemistry and Biochemistry,University of Windsor, 3Department of Physics,University of Windsor

Chapters

  • 00:04Introduction
  • 00:51Setting Up and Initiating the Stopped-Flow System
  • 02:05Loading the Sample and Priming the System
  • 03:01Defining the Stopped-Flow Mixing Protocol in the Program Script
  • 03:43Defining Small-Angle Neutron Scattering (SANS) Parameters in the Instrument Control GUI
  • 04:05Collecting Standard Scattering Measurements for Data Reduction Before Beginning the Stopped-Flow Experiment to Process the Time Resolved-SANS Data
  • 04:47Beginning the Stopped-Flow Experiment
  • 05:29Removing All Backgrounds, Correcting for Detector Sensitivity, Correcting for Sample Transmission,and Analyzing the Time Resolved-SANS Data
  • 06:08Results: Raw Neutron Data and Scattered Intensities of Catalyzed Exchange of Outer Layer of Lipid Vesicles at Various Temperatures
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents the use of a stopped-flow sample environment to quickly mix multiple liquid solutions in situ during a small-angle neutron scattering measurement and to study kinetic processes on nanometer length scales and second time scales.

Tags

Keywords: Stop FlowSmall Angle Neutron ScatteringSANSNanoscale MaterialsTime-evolutionHydrogenDeuteriumNeutron ScatteringLipidsProteinsPolymersNISTNCNRPumpSyringeTubingValvesMixerSample CellSample LoadingPriming

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