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Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
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Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Article DOI: 10.3791/62880-v • 09:31 min • September 17th, 2021
September 17th, 2021 •
1,3, 1,4, 1,2,5, 1,6
1Molecular Microbial Pathogenesis Unit, Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses, Collège de France, 3Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy, Université Paris-Saclay, 5The Center for Microbes, Development and Health, Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit, Institut Pasteur

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Summary
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The present protocol describes a method to detect reactive oxygen species (ROS) in the intestinal murine organoids using qualitative imaging and quantitative cytometry assays. This work can be potentially extended to other fluorescent probes to test the effect of selected compounds on ROS.

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Oxidative Stress Murine Intestinal Organoids Reactive Oxygen Species Fluorogenic Probe Cellular Biology ROS Levels Living Primary Cells In Vitro Microscopy Flow Cytometry 96-well Plates Organoid Models Fluorescent Sensors Organoid Culture Passaging Screening Approaches Lgr5-GFP Mouse Jejunum Duodenum Ileum DPBS Antibiotics Ball Tip Scissors Petri Dish
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