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JoVE Journal Neuroscience
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Tessuto trattato con radioligandi attivati da fluorescenza per determinare l'origine cellulare del segnale radioattivo

Article DOI: 10.3791/62883-v • 12:04 min • September 10th, 2021
September 10th, 2021 •
1,2, 1,3,4, 1,5, 1,2, 1,2
1Division of Adult Psychiatry, Department of Psychiatry, University Hospitals of Geneva, 2Department of Psychiatry, University of Geneva, 3Division of Nuclear medicine and Molecular Imaging, University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology, University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine, Imperial College London

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Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) è un potente strumento per studiare il ruolo della proteina traslocatore 18 kDa o dell'espressione del recettore della serotonina 5HT2A nella malattia di Alzheimer su scala cellulare. Questo protocollo descrive l'applicazione ex-vivo di FACS-RTT nel modello di ratto TgF344-AD.

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Neuroscienze Numero 175 Morbo di Alzheimer Selezione cellulare attivata da fluorescenza a tessuti a reticolari radioligidali TgF344-AD Tomografia a emissione di positroni/Tomografia computerizzata a emissione di fotoni singoli TSPO 5HT2AR
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