Journal
/
Medicine
/
Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome

Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome

DOI:
10.3791/62888-v

•

07:38 min

•

August 03, 2021

•

, , , , , , ,
1Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital, 2Department of Molecular Biology & Biochemistry,University of California, Irvine, 3Department of Medicine,Massachusetts General Hospital, 4Department of Microbiology & Immunology,Geisel School of Medicine at Dartmouth, 5Swiss i-research and teaching institute

Chapters

  • 00:04Introduction
  • 00:51Artificial Sputum Medium Preparation
  • 02:28Oxygen Sparging
  • 05:25Results: Culturing in Artificial Sputum Medium
  • 07:09Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to develop a model system for the effect of hyperoxia on cystic fibrosis airway microbial communities. Artificial sputum medium emulates the composition of sputum, and hyperoxic culture conditions model the effects of supplemental oxygen on lung microbial communities.

Tags

Keywords: Cystic FibrosisAirway MicrobiomeSupplemental OxygenArtificial Sputum MediumASCMASMMASBMMops BufferPHFiltrationVacuumOrbital ShakerCold RoomOxygen SpargingSerum BottleMetagenomics Sequencing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62888?article_type=v

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy
check_url/62888?article_type=v

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD
check_url/62888?article_type=v

The Dimethylnitrosamine Induced Liver Fibrosis Model in the Rat
check_url/62888?article_type=v

Topical Airway Anesthesia for Awake-endoscopic Intubation Using the Spray-as-you-go Technique with High Oxygen Flow
check_url/62888?article_type=v

A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
check_url/62888?article_type=v

Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
check_url/62888?article_type=v

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography
check_url/62888?article_type=v

A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
check_url/62888?article_type=v

Culture and Imaging of Human Nasal Epithelial Organoids
check_url/62888?article_type=v

Rectal Organoid Morphology Analysis (ROMA): A Diagnostic Assay in Cystic Fibrosis

Read Article