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Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome

Article DOI: 10.3791/62888-v • 07:38 min • August 3rd, 2021
August 3rd, 2021 •
1, 2, 1, 3, 2, 4, 5, 1
1Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital, 2Department of Molecular Biology & Biochemistry, University of California, Irvine, 3Department of Medicine, Massachusetts General Hospital, 4Department of Microbiology & Immunology, Geisel School of Medicine at Dartmouth, 5Swiss i-research and teaching institute

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The goal of this protocol is to develop a model system for the effect of hyperoxia on cystic fibrosis airway microbial communities. Artificial sputum medium emulates the composition of sputum, and hyperoxic culture conditions model the effects of supplemental oxygen on lung microbial communities.

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Supplemental Oxygen Cystic Fibrosis Airway Microbiome In Vitro Model Lung Microbiome Sputum Composition Sparging Process Artificial Sputum Medium Chronic Lung Diseases Glucose Concentrations Cystic Fibrosis And Diabetes Medium Preparation ASCM ASMM ASBM Mops Buffer PH Titration Filter Sterilization Cold Room Orbital Shaker Filtration Process
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