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High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
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High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Article DOI: 10.3791/62893-v • 07:28 min • October 4th, 2021
October 4th, 2021 •
1, 1, 1, 1
1Department of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach

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A high-throughput protocol for the surface sterilization of Arabidopsisthaliana (Arabidopsis) seeds is provided, optimizing the liquid handling steps with a simple suction device constructed with a vacuum pump. Hundreds of seed samples can be surface-sterilized in one day.

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High-throughput Robust Time-flexible Surface Sterilization Arabidopsis Seeds Functional Genomics Model Species Ease High Throughput Hundreds Of Samples Per Day Modifications Model And Non-model Plant Species Temperature Medium Centrifugation Speed Seed Survival 70% Ethanol 5% Bleach Solution Nonionic Detergent Half-strength Murashige And Skoog Medium MS Medium Powder Sucrose PH Adjustment Potassium Hydroxide Distilled Water Autoclaving Solid Medium Petri Dishes
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