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Måling mitokondrie substrat flux i rekombinant perfringolysin O-Permeabilized celler
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Måling mitokondrie substrat flux i rekombinant perfringolysin O-Permeabilized celler

Article DOI: 10.3791/62902-v • 06:17 min • August 13th, 2021
August 13th, 2021 •
1,2, 1,2, 1, 1, *2,3, *1
1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady
* These authors contributed equally

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I dette arbejde beskriver vi en modificeret protokol til test af mitokondriesubstratstrømpe ved hjælp af rekombinant perfringolysin O i kombination med mikropladebaseret respirometri. Med denne protokol viser vi, hvordan metformin påvirker mitokondrie respiration af to forskellige tumorcellelinjer.

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Biologi Udgave 174 mikroplade respirometri mitokondrier metformin permeabiliserede celler perfringolysin O mitokondriesubstrater
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