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Meten van mitochondriale substraatflux in recombinante perfringolysine O-permeabiliseerde cellen
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Meten van mitochondriale substraatflux in recombinante perfringolysine O-permeabiliseerde cellen

Article DOI: 10.3791/62902-v • 06:17 min • August 13th, 2021
August 13th, 2021 •
1,2, 1,2, 1, 1, *2,3, *1
1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady
* These authors contributed equally

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In dit werk beschrijven we een aangepast protocol om mitochondriale respiratoire substraatflux te testen met behulp van recombinant perfringolysine O in combinatie met respirometrie op basis van microplaten. Met dit protocol laten we zien hoe metformine de mitochondriale ademhaling van twee verschillende tumorcellijnen beïnvloedt.

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Biologie Nummer 174 microplaat respirometrie mitochondriën metformine permeabiliseerde cellen perfringolysine O mitochondriale substraten
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