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Måling av mitokondrie substrat flux i rekombinante perfringolysin O-permeabiliserte celler
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Måling av mitokondrie substrat flux i rekombinante perfringolysin O-permeabiliserte celler

Article DOI: 10.3791/62902-v • 06:17 min • August 13th, 2021
August 13th, 2021 •
1,2, 1,2, 1, 1, *2,3, *1
1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady
* These authors contributed equally

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I dette arbeidet beskriver vi en modifisert protokoll for å teste mitokondrie substratfluks ved hjelp av rekombinant perfringolysin O i kombinasjon med mikroplatebasert respirometri. Med denne protokollen viser vi hvordan metformin påvirker mitokondrie respirasjon av to forskjellige tumorcellelinjer.

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Biologi utgave 174 mikroplate respirometri mitokondrier metformin permeabiliserte celler perfringolysin O mitokondrie substrater
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