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JoVE Journal Biochemistry
Tidsavklarte Förster Resonance Energy Transfer Assays for måling av endogene fosforylaterte STAT-proteiner i humane celler
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Tidsavklarte Förster Resonance Energy Transfer Assays for måling av endogene fosforylaterte STAT-proteiner i humane celler

Article DOI: 10.3791/62915-v • 07:12 min • September 9th, 2021
September 9th, 2021 •
1, 1, 1
1BioAuxilium Research

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Tidsavklarte Förster-resonansenergioverføringscellebaserte analyseprotokoller er beskrevet for enkel, spesifikk, sensitiv og robust kvantifisering av endogen fosforylater og aktivator for transkripsjon (STAT) 1/3/4/5/6 proteiner i cellelysater i et 384-brønns format.

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Biokjemi Utgave 175 JAK/STAT-signalering STAT-proteiner Fosforylering Homogene analyser TR-FRET Immunoassay Cellebasert analyse Kinases Cellesignalering Screening med høy gjennomstrømning Cytokinsignalering
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