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Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field
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JoVE Journal Biology
Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field

Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field

DOI:
10.3791/62956-v

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08:54 min

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August 27, 2021

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, , , , , ,
1School of Kinesiology,Auburn University, 2Department of Biological Sciences,Auburn University, 3Department of Electrical and Computer Engineering,Auburn University

Chapters

  • 00:04Introduction
  • 00:56Isolation of Mitochondria
  • 03:53Mitochondrial Respiration Measurements
  • 06:47Results: Mitochondrial Respiration Rates from Wild-Derived House Mice (Mus musculus) Hindlimb Skeletal Muscle
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

We designed and constructed a mobile laboratory to measure respiration rates in isolated mitochondria of wild animals captured at field locations. Here, we describe the design and outfitting of a mobile mitochondrial laboratory and the associated laboratory protocols.

Tags

Keywords: Mobile Mitochondrial LaboratoryMitochondrial EnergeticsMitochondrial RespirationField PhysiologyEcological And Evolutionary ContextMitochondrial IsolationMitochondrial Respiration ChambersMobile Lab Setup

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