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Journal / Bioengineering / Visualização da dinâmica plus-end de microtúbulo…
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Bioengineering
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JoVE Journal Bioengineering
Visualização da dinâmica plus-end de microtúbulo no modelo de doença de Huntington baseado em fibroblastos de pele primárias humanas
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Visualização da dinâmica plus-end de microtúbulo no modelo de doença de Huntington baseado em fibroblastos de pele primárias humanas

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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Este protocolo é dedicado à visualização plus-end do microtúbulo pela transfecção de proteínaS EB3 para estudar suas propriedades dinâmicas na cultura celular primária. O protocolo foi implementado em fibroblastos de pele primários humanos obtidos de pacientes com a doença de Huntington.

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Bioengenharia Edição 179 microtúbulos transfecção EB3 cultura primária doença de Huntington microscopia vitalícia
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