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JoVE Journal
Developmental Biology

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An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila
 

An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

Article DOI: 10.3791/62983-v 07:06 min October 13th, 2021
October 13th, 2021

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Summary

This protocol describes the dissection procedure, culture condition, and live imaging of an antennae-brain explant system for the study of the olfactory circuit assembly.

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Tags

Explant System Time-lapse Imaging Olfactory Circuit Assembly Drosophila Protocol Live Imaging Single Axon Level Developing Brain Dissection High Quality Images Cell Biological Basis Circuit Development Culture Dish Sylgard Immobilizing The Explant Micro Pins Forceps Pupae Water Bath ORN Clones
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