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Automating Aggregate Quantification in Caenorhabditis elegans
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Automating Aggregate Quantification in Caenorhabditis elegans

Automating Aggregate Quantification in Caenorhabditis elegans

DOI:
10.3791/62997-v

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07:50 min

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October 14, 2021

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, , , , ,
1Department of Microbiology and Cell Science,University of Florida, 2Department of Computer and Information Science and Engineering,University of Florida

Chapters

  • 00:04Introduction
  • 02:28Image Analysis
  • 05:38Results: The Analysis of Aggregate Detection and the Aggregate Quantification Efficacy Using CellProfiler and Automation

Summary

Automatic Translation

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The following protocol describes the development and optimization of a high-throughput workflow for worm culturing, fluorescence imaging, and automated image processing to quantify polyglutamine aggregates as an assessment of changes in proteostasis.

Tags

Keywords: Polyglutamine AggregationProteostasisCaenorhabditis ElegansAutomated Aggregate CountingHigh-throughputMicroscopy ImagingCellProfilerImage Analysis PipelineUntangled Worms ModuleBacterial GenesNeurodegenerative Diseases

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