2,711 Views
•
07:50 min
•
October 14, 2021
DOI:
Summary
Automatically generated
The following protocol describes the development and optimization of a high-throughput workflow for worm culturing, fluorescence imaging, and automated image processing to quantify polyglutamine aggregates as an assessment of changes in proteostasis.
Explore More Videos
Related Videos
11:58
The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
Related Videos
9379 Views
10:23
Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion
Related Videos
9793 Views
12:22
Automated Quantification of Synaptic Fluorescence in C. elegans
Related Videos
10326 Views
05:51
Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease
Related Videos
2688 Views
06:49
Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
Related Videos
2785 Views
10:58
Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
Related Videos
17708 Views
07:20
Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform
Related Videos
9114 Views
09:06
Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans
Related Videos
7447 Views
08:47
Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
Related Videos
9627 Views
N/A
Quantification of Insoluble Protein Aggregation in Caenorhabditis elegans during Aging with a Novel Data-Independent Acquisition Workflow
Related Videos
2279 Views
Read Article
Cite this Article
Vaziriyan-Sani, A. S., Handy, R. D., Walker, A. C., Pagolu, C. N., Enslow, S. M., Czyż, D. M. Automating Aggregate Quantification in Caenorhabditis elegans. J. Vis. Exp. (176), e62997, doi:10.3791/62997 (2021).
Copy