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Rilevamento del virus e delle proteine salivari di un vettore cicalina nell'ospite della pianta
JoVE Journal
Biology
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JoVE Journal Biology
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
DOI:

07:23 min

September 14, 2021

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Chapters

  • 00:05Introduction
  • 00:35Non-Viruliferous Insect Rearing
  • 01:29Virus Acquisition and Collection of Viruliferous Insects
  • 02:10Collection of Salivary Proteins Using a Feeding Cage
  • 03:04Reagent Preparation
  • 03:51Detection of Saliva and Viral Proteins by Western Blotting
  • 06:02Results: Western Blot Analysis for Detection of Viral and Salivary Proteins of a Leafhopper Vector in the Plant Host
  • 06:51Conclusion

Summary

Automatic Translation

Questo protocollo dimostra come utilizzare l'ospite della pianta per rilevare le proteine salivari delle cicaline e le proteine virali vegetali rilasciate dai vettori delle cicaline.

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