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Journal
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Developmental Biology
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マウスとヒトの歯からの単一細胞の迅速な分離
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Rapid Isolation of Single Cells from Mouse and Human Teeth
Please note that all translations are automatically generated.
Click here for the English version.
マウスとヒトの歯からの単一細胞の迅速な分離
DOI:
10.3791/63043-v
•
06:09 min
•
October 28, 2021
•
Jan Krivanek
,
Josef Lavicky
,
Thibault Bouderlique
,
Igor Adameyko
3
1
Department of Histology and Embryology, Faculty of Medicine
,
Masaryk University
,
2
Department of Molecular Neuroimmunology, Centre for Brain Research
,
Medical University of Vienna
,
3
Department of Physiology and Pharmacology
,
Karolinska Institute
Chapters
00:05
Introduction
01:09
Tissue Dissection
02:58
Single-Cell Suspension Preparation
04:42
Results: Quantification of the Immune Cells
05:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
現在のプロトコルは、成長し続けるマウス切歯、マウス臼歯、およびヒトの歯から単細胞RNA-seq分析に適した単一細胞を単離するための迅速で効率的で穏やかな方法を提示します。
Tags
Single-cell Isolation
Mouse Teeth
Human Teeth
Cell Suspension
Dental Pulp
Mandible
Euthanasia
HBSS
Tweezers
Scalpel
Cell Quality
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