A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Pathology,University of Michigan, Ann Arbor, 2Department of Internal Medicine,University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology,University of Michigan, Ann Arbor, 4Rogel Cancer Center,University of Michigan, Ann Arbor, 5Institute of Gerontology,University of Michigan, Ann Arbor
Chapters
- 00:05Introduction
- 01:08Hydration of the Sensor Cartridge
- 02:03Preparation of Cell-Adhesive-Coated Microplates
- 02:52Seeding Cells in Cell-Adhesive-Coated Microplates
- 04:10Glycolysis Stress Test Using the Extracellular Flux Analyzer
- 07:53Results: Assessment of the Glycolytic Function and Mitochondrial Respiration in Mouse HSPCs
- 09:36Conclusion
The method presented here summarizes optimized protocols for assessing cellular bioenergetics in non-adherent mouse hematopoietic stem and primitive progenitor cells (HSPCs) using the extracellular flux analyzer to measure the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of HSPCs in real time.
Tags
Keywords:
Hematopoietic Stem CellsHematopoiesisGlycolysisOxidative PhosphorylationCellular BioenergeticsExtracellular Flux AnalyzerMetabolic SwitchMouse Hematopoietic Stem And Progenitor CellsHigh Throughput QuantificationMetabolic PathwaysPluripotencySuspension CellsCell Adhesive Solution96-well Microplate
