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Vurdering af cellulær bioenergetik i hæmatopoietisk stamme og primitive stamceller ved hjælp af den ekstracellulære fluxanalysator
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Vurdering af cellulær bioenergetik i hæmatopoietisk stamme og primitive stamceller ved hjælp af den ekstracellulære fluxanalysator

Article DOI: 10.3791/63045-v • 10:17 min • September 24th, 2021
September 24th, 2021 •
1, 2, 2,3,4, 1,4,5
1Department of Pathology, University of Michigan, Ann Arbor, 2Department of Internal Medicine, University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology, University of Michigan, Ann Arbor, 4Rogel Cancer Center, University of Michigan, Ann Arbor, 5Institute of Gerontology, University of Michigan, Ann Arbor

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Metoden, der præsenteres her, opsummerer optimerede protokoller til vurdering af cellulær bioenergetik i ikke-klæbende musehæmatopoietisk stamme og primitive stamceller (HSPC'er) ved hjælp af den ekstracellulære fluxanalysator til måling af den ekstracellulære forsuringshastighed (ECAR) og iltforbrugshastighed (OCR) for HSPC'er i realtid.

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Biologi udgave 175 hæmatopoietiske stamceller hæmatopoietiske stamceller glykolyse mitokondriel respiration oxidativ phosphorylering ekstracellulær forsuringshastighed iltforbrugshastighed ekstracellulær flux ikke-klæbende celler
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