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Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

DOI:
10.3791/63048-v

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07:28 min

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November 04, 2021

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, , ,
1Tim Taylor Department of Chemical Engineering,Kansas State University, 2Department of Materials Science and Engineering,The Pennsylvania State University

Chapters

  • 00:04Introduction
  • 00:42Hydrogel Formation on Glass Coverslip
  • 01:57Hydrogel Formation Over Microwell Arrays
  • 03:00Hydrogel Degradation with the Patterned Illumination Tool
  • 04:13Cell Retrieval
  • 04:55Results: Cell Viability Assessment
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

The use of photodegradable hydrogels to isolate bacterial cells by utilizing a high-resolution light pattering tool is reported. Essential experimental procedures, results, and advantages of the process are reviewed. The method enables rapid and inexpensive isolation of targeted bacteria showing rare or unique functions from heterogeneous communities or populations.

Tags

Keywords: Photodegradable HydrogelBacterial ScreeningCell IsolationMicrofluidicsCell EncapsulationHydrogel PrecursorPEG DiacrylatePEG ThiolMicrowell ArrayATGN MediaCell SuspensionPhosphate Buffered Saline

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