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Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
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Bioengineering
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JoVE Journal Bioengineering
Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro

Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro

DOI:
10.3791/63065-v

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14:49 min

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April 15, 2022

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, , ,
1Materials Science and Engineering Program,University of California, Riverside, 2Department of Bioengineering,University of California, Riverside, 3Stem Cell Center,University of California, Riverside

Chapters

  • 00:07Introduction
  • 02:04Cell Culture Preparation
  • 02:20Harvesting Bone Marrow Derived Mesenchymal Stem Cells (BMSCs) from Rat Weanlings
  • 04:04Sample Preparation and Sterilization
  • 04:25Cell Culture Methods
  • 06:52Postculture Characterization of Cells
  • 10:22Postculture Analyses of Media and Samples
  • 11:55Representative Results
  • 14:06Conclusions

Summary

Automatic Translation

Automatic Translation

We introduce three methods of direct culture, direct exposure culture, and exposure culture for evaluating the in vitro cytocompatibility of biodegradable implant materials. These in vitro methods mimic different in vivo cell-implant interactions and can be applied to study various biodegradable materials.

Tags

Keywords: Biodegradable ImplantsIn Vitro Cell CultureCytocompatibilityCytotoxicityDirect CultureDirect Exposure CultureExposure CultureMesenchymal Stem CellsBiomedical Applications

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