Journal
/
Medicine
/
Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma
JoVE Journal
Medicine
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

DOI:
10.3791/63083-v

•

07:53 min

•

April 15, 2022

•

, , , , , , , , ,
1Department of Hematology,Zhongnan Hospital of Wuhan University, 2Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology

Chapters

  • 00:09Introduction
  • 01:23Preparation of Bone Marrow Smears
  • 02:37FISH Pre-Processing of Bone Marrow Smear
  • 03:48Bone Marrow Smear FISH and Washing
  • 05:20FISH Analysis and Imaging
  • 06:20Representative Result
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for improving the success of interphase fluorescence in situ hybridization detection on bone marrow smears from multiple myeloma patients.

Tags

InterphaseFluorescence In Situ HybridizationBone Marrow SmearsMultiple MyelomaPlasma CellsCIg FISHGenetic Risk StratificationWright-Giemsa StainingMicroscopyFixative SolutionSSC Buffer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia

Femoral Bone Marrow Aspiration in Live Mice

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Read Article