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The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
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The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

DOI:
10.3791/63087-v

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08:51 min

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February 12, 2022

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,
1Department of Microbiology and Cell Biology,Indian Institute of Science, Bangalore KA 560012, India

Chapters

  • 00:06Introduction
  • 01:04Seeding of Mouse Melanocytes on Pre-Treated Coverslips
  • 01:42Transfection of Cells with the STX13 Plasmids
  • 02:39Fixation of the Cells
  • 03:11Immunostaining of the Cells
  • 05:05Results: Quantification of Overlap Between the RE Localized Proteins and Melanosomes
  • 07:25Results: Quantification of Recycling Endosomes’ Tublar Number and Length
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Recycling endosomes are part of the endosomal tubular network. Here we present a method to quantify the dynamics of recycling endosomes using GFP-STX13 as an organelle marker.

Tags

Microscopy-based AssayRecycling EndosomesSNARE TraffickingMelanocytesMelanosomesLysozome Related Organelles (LRO)Syntaxin-13TransfectionBasement Membrane MatrixPMAOPTI-MEMRPMI Medium

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