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Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma
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JoVE Journal Cancer Research
Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma

DOI:
10.3791/63091-v

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09:06 min

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November 11, 2021

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, , ,
1Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children’s Hospital,IRCCS, 2Oncology Service,Children’s Health Queensland Hospital and Health Service, 3Child Health Research Centre,The University of Queensland

Chapters

  • 00:05Introduction
  • 01:04ALR/Antibody Complex Preparation and Invasion Assay Setup
  • 03:25Migration Assay Setup
  • 04:18Live-Cell Analysis Instrument Setting for Image Acquisition
  • 05:14Live-Cell Analysis Instrument Setting for Image Analysis
  • 06:30Results: Analysis of CD44 Expression in 3D Tumor Cell Invasion and Migration
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

This study presents a protocol of live-3D-cell immunocytochemistry applied to a pediatric diffuse midline glioma cell line, useful to study in real-time the expression of proteins on the plasma membrane during dynamic processes like 3D cell invasion and migration.

Tags

Keywords: Live Cell Imaging3D Cell CultureImmunocytochemistryDiffuse Midline GliomaCD44Cell MigrationCell InvasionMolecular Target

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