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Immunology and Infection

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JoVE Journal Immunology and Infection
Live imaging og kvantificering af virusinfektion i K18 hACE2 transgene mus ved hjælp af reporter-udtrykkende rekombinant SARS-CoV-2
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Live imaging og kvantificering af virusinfektion i K18 hACE2 transgene mus ved hjælp af reporter-udtrykkende rekombinant SARS-CoV-2

Article DOI: 10.3791/63127-v • 08:41 min • November 5th, 2021
November 5th, 2021 •
1, 1, 1, 1, 1, 1
1Texas Biomedical Research Institute

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Denne protokol beskriver dynamikken i virusinfektioner ved hjælp af luciferase- og fluorescensekspressive rekombinante (r) SARS-CoV-2 og et in vivo-billeddannelsessystem (IVIS) i K18 hACE2 transgene mus for at overvinde behovet for sekundære tilgange, der kræves for at studere SARS-CoV-2-infektioner in vivo.

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Immunologi og infektion udgave 177 SARS-CoV-2 coronavirus fluorescens bioluminescens replikationskompetente vira reportervirus NanoLuc luciferase venus fluorescerende protein virusinfektion in vivo-billeddannelse IVIS Ami HT K18 hACE2 transgene mus
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