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Live imaging og kvantificering af virusinfektion i K18 hACE2 transgene mus ved hjælp af reporter-udtrykkende rekombinant SARS-CoV-2
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
DOI:

08:41 min

November 05, 2021

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Chapters

  • 00:05Introduction
  • 01:15Bioluminescence Monitoring K18 hACE2 Transgenic Mice Infected with rSARS-CoV-2/Nluc
  • 02:24Fluorescence Analysis in K18 hACE2 Transgenic Mice Infected with rSARS-CoV-2/Venus
  • 03:49Viral Titrations
  • 05:48Results: rSARS-CoV-2Nluc and rSARS-CoV-2/Venus Expression in Infected K18 Hace2 Transgenic Mice
  • 08:12Conclusion

Summary

Automatic Translation

Denne protokol beskriver dynamikken i virusinfektioner ved hjælp af luciferase- og fluorescensekspressive rekombinante (r) SARS-CoV-2 og et in vivo-billeddannelsessystem (IVIS) i K18 hACE2 transgene mus for at overvinde behovet for sekundære tilgange, der kræves for at studere SARS-CoV-2-infektioner in vivo.

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