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DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
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Chapters
- 00:04Introduction
- 00:34Primary Antibody Incubation
- 01:29Sample Washes, Secondary Antibody Incubation, Ligation, Amplification, and Mounting
- 03:49Digital Imaging with a Confocal Microscope
- 05:49Image Analysis
- 07:41Results: Increased Synapse Formation by Rapid Antidepressants in the Presence of GABAB Agonists
- 08:42Conclusion
DetectSyn is an unbiased, rapid fluorescent assay that measures changes in relative synapse (pre- and postsynaptic engagement) number across treatments or disease states. This technique utilizes a proximity ligation technique that can be used both in cultured neurons and fixed tissue.
