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Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
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Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Article DOI: 10.3791/63143-v • 08:30 min • December 8th, 2021
December 8th, 2021 •
1, 1
1Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University

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Summary
Automatic Translation

This protocol aims to isolate cell-type-specific translating ribosomal mRNAs using the NuTRAP mouse model.

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Isolate Cell-type-specific RNAs Snap-frozen Heterogeneous Tissue Samples Cell Sorting Ribosome Bound RNAs GLP Pull Down EGFP Expressing Cells Homogenization Working Buffer Glass Tissue Grinder Set Centrifuge Anti-GFP Antibody End Over End Rotator Protein G Beads Magnetic Rack Negative Fraction
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