Journal
/
Developmental Biology
/
Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

DOI:
10.3791/63160-v

•

07:14 min

•

October 19, 2021

•

,
1Department of Molecular Genetics,Weizmann Institute of Science

Chapters

  • 00:04Introduction
  • 00:47Ex Utero Roller Culture of Embryos from Embryonic Day 7.5 to 11
  • 03:48Extended Embryo Culture from Embryonic Day 5.5/6.5 to 11
  • 04:38Results: Continuous and Robust Ex Utero Development of Post Implantation Mouse Embryos
  • 06:34Conclusion

Summary

Automatic Translation

Automatic Translation

An enhanced platform for whole-embryo culture allows continuous and robust ex utero development of postimplantation mouse embryos for up to six days, from pregastrulation stages until advanced organogenesis. In this protocol, we detail the standard procedure for successful embryo culture using static plates and rotating bottle systems.

Tags

Keywords: Ex Utero CultureMouse EmbryosPregastrulationOrganogenesisGastrulationRoller Culture IncubatorGas ConcentrationGas PressureEmbryo DissectionCulture MediumDPBSPregnant Mouse

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63160?article_type=v

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development
check_url/63160?article_type=v

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow
check_url/63160?article_type=v

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
check_url/63160?article_type=v

Ex Vivo Culture of Pharyngeal Arches to Study Heart and Muscle Progenitors and Their Niche
check_url/63160?article_type=v

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
check_url/63160?article_type=v

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
check_url/63160?article_type=v

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
check_url/63160?article_type=v

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
check_url/63160?article_type=v

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue
check_url/63160?article_type=v

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Read Article