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Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
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Biochemistry
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JoVE Journal Biochemistry
Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

DOI:
10.3791/63164-v

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14:39 min

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April 22, 2022

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, , , ,
1Department of Chemical Biological Sciences,Universidad Autónoma de Ciudad Juárez

Chapters

  • 00:42Introduction
  • 02:58Sample Preparation
  • 04:37UPLC-MS/MS Analysis
  • 06:36Database Construction
  • 09:49Data Analysis
  • 11:25Representative Results
  • 14:13Conclusions

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to detect phenolic metabolites in plasma using a semi-targeted chromatography-mass spectrometry method.

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Keywords: Semi-targetedUltra-high-performance ChromatographyMass SpectrometryPhenolic MetabolitesPlasmaElderly AdultsUPLC-MS/MSProtein PrecipitationMetabolite IdentificationBrosimum AlicastrumBioavailabilityBiotransformationUntargeted AnalysisSemi-targeted AnalysisMolecular FeaturesMass-to-charge RatioIsotopic DistributionFragmentation PatternPolyphenol Metabolites

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