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Biological Preparation and Mechanical Technique for Determining Viscoelastic Properties of Zonular Fibers
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JoVE Journal Medicine
Biological Preparation and Mechanical Technique for Determining Viscoelastic Properties of Zonular Fibers

Biological Preparation and Mechanical Technique for Determining Viscoelastic Properties of Zonular Fibers

DOI:
10.3791/63171-v

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06:39 min

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December 16, 2021

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, , ,
1Department of Basic Sciences,University of Health Sciences and Pharmacy, 2Department of Ophthalmology & Visual Sciences,Washington University School of Medicine, 3Department of Biomedical Engineering,The Ohio State University, 4Department of Cell Biology & Physiology,Washington University School of Medicine

Chapters

  • 00:05Introduction
  • 01:00Sample Preparation and Data Acquisition
  • 04:58Results: Representative Results for Viscoelastic Properties of Zonular Fibers in Wild-Type and MAGP-1 Deficient Mice
  • 05:57Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes a method for the study of extracellular matrix viscoelasticity and its dependence on protein composition or environmental factors. The matrix system targeted is the mouse zonule. The performance of the method is demonstrated by comparing the viscoelastic behavior of wild-type zonular fibers with those lacking microfibril-associated glycoprotein-1.

Tags

Keywords: Viscoelastic PropertiesZonular FibersEyeLensMechanical TechniqueBiological PreparationMouse ModelGene MutationsMicrofibril ProteinsInherited Syndromic ConditionsFixationOphthalmic SurgeryStereo MicroscopeInstant GluePetri Dish

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