Journal
/
Biochemistry
/
Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth

DOI:
10.3791/63191-v

•

09:10 min

•

January 07, 2022

•

, ,
1Department of Science and Technology,Bryant University, 2Center for Health and Behavioral Sciences,Bryant University

Chapters

  • 00:04Introduction
  • 00:35Preparation of Masarimycin
  • 04:57Morphological Study
  • 07:38Results: Masarimycin as a Chemical Probe in Synergy/Antagonism Assays and Morphological Studies of Gram-Positive Microorganisms
  • 08:40Conclusion

Summary

Automatic Translation

Automatic Translation

A detailed protocol is presented for preparing the bacteriostatic diamide masarimycin, a small molecule probe that inhibits the growth of Bacillus subtilis and Streptococcus pneumoniae by targeting cell wall degradation. Its application as a chemical probe is demonstrated in synergy/antagonism assays and morphological studies with B. subtilis and S. pneumoniae.

Tags

MasarimycinGram-positive BacteriaAutolysin InhibitorBacterial Growth InhibitorOrganic SynthesisCyclohexylamineCyclohexyl CarboxaldehydeOrtho-iodobenzoic AcidCyclohexyl IsocyanideMethanolThin-layer ChromatographyEthyl AcetateHydrochloric AcidSodium BicarbonateSodium ChlorideSodium Sulfate

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Read Article