Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / High-throughput Confocal Imaging of Quantum…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae
Journal (Biology)
High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae
Isolation of Human Primary Valve Cells for In vitro Disease Modeling
Journal (Biology)
Isolation of Human Primary Valve Cells for In vitro Disease Modeling
 

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Article DOI: 10.3791/63202-v • 06:39 min • April 21st, 2022
April 21st, 2022 •
1, 2, 2,3, 2, 1
1National Center for Advancing Translational Sciences, 2Optical Sciences Division, Code 5600, Naval Research Laboratory, 3Jacobs Corporation

Chapters

Summary
Automatic Translation

In this protocol, quantum dots conjugated to recombinant SARS-CoV-2 spike enable cell-based assays to monitor spike binding to hACE2 at the plasma membrane and subsequent endocytosis of the bound proteins into the cytoplasm.

Tags

High-throughput Confocal Imaging Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers Binding Endocytosis HEK293T Cells Platform Technology Spike Mediated Cell Recognition Cellular Viral Infection Quantum Dots Functionalized Surface Visualize Measure Internalization BSA Serial Dilution Triplicate Concentration Nanomole Multi-channel Aspirator Multi-channel Pipette Imaging Media Incubator Carbon Dioxide Protective Equipment PFA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter