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In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

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08:16 min

December 29, 2021

DOI:

08:16 min
December 29, 2021

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Summary

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This protocol describes a battery of methods that includes analytical size-exclusion chromatography to study histone chaperone oligomerization and stability, pull-down assay to unravel histone chaperone-histone interactions, AUC to analyze the stoichiometry of the protein complexes, and histone chaperoning assay to functionally characterize a putative histone chaperone in vitro.

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