JoVE Journal
Immunology and Infection
Immunology and Infection
A subscription to JoVE is required to view this content.
Chapters
Summary
Here, we describe the construction of null mutants of Aeromonas in specific glycosyltransferases or regions containing glycosyltransferases, the motility assays, and flagella purification performed to establish the involvement and function of their encoded enzymes in the biosynthesis of a glycan, as well as the role of this glycan in bacterial pathogenesis.