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DOI: 10.3791/63246-v
This study explores the mass production of infective conidia from South African isolates of the entomopathogenic fungi Metarhizium robertsii and M. pinghaense for use in biological control against agricultural insect pests. The findings contribute to integrated pest management strategies amid growing restrictions on conventional insecticides due to environmental and health concerns.
Entomopathogenic fungi have gained importance as the biological control agents of agricultural insect pests. In this study, the mass production of a sufficient number of resilient infective propagules of South African isolates of both Metarhizium robertsii and M. pinghaense for commercial application against insect pests was successfully conducted using agricultural grain products.
This method provides new information on how to mass produce infective conidia of entomopathogenic fungi to control important insect pests in commercial agro-ecosystems, given the current situation where most insecticides are phased out due to concerns of their toxic effect on the environment and human health. The technique describes the method used to ensure the optimum yield of EPF conidia during mass production. The method is helpful for integrated pest management and biological control by providing insight into the effectiveness of mass produced conidia for large-scale management of damaging insecticide resistance insect pests in the agro-ecosystems.
This procedure was developed and demonstrated by Dr.Letodi Luki Mathulwe, Dr.Nomakholwa Faith Stokwe, and Professor Antoinette Paula Malan. Heat one liter distilled water and switch off the heat before reaching the boiling point. Now, add 30 grams of glucose, four grams of potassium phosphate dibasic, 20 grams of yeast extract, 15 milliliters of corn steep liquor, and bring the contents to a gentle boil for two to three minutes.
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