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Biology
Mass Production of Entomopathogenic Fungi, Metarhizium robertsii and Metarhizium pin...
Mass Production of Entomopathogenic Fungi, Metarhizium robertsii and Metarhizium pin...
JoVE Journal
Biology
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JoVE Journal Biology
Mass Production of Entomopathogenic Fungi, Metarhizium robertsii and Metarhizium pinghaense, for Commercial Application Against Insect Pests

Mass Production of Entomopathogenic Fungi, Metarhizium robertsii and Metarhizium pinghaense, for Commercial Application Against Insect Pests

Full Text
8,221 Views
10:10 min
March 31, 2022

DOI: 10.3791/63246-v

Letodi Luki Mathulwe1, Antoinette Paula Malan1, Nomakholwa Faith Stokwe1

1Department of Conservation Ecology and Entomology, Faculty of AgriSciences,Private Bag X1

Overview

This study explores the mass production of infective conidia from South African isolates of the entomopathogenic fungi Metarhizium robertsii and M. pinghaense for use in biological control against agricultural insect pests. The findings contribute to integrated pest management strategies amid growing restrictions on conventional insecticides due to environmental and health concerns.

Key Study Components

Research Area

  • Biological control agents in agriculture
  • Entomopathogenic fungi
  • Integrated pest management

Background

  • Concerns regarding the toxic effects of synthetic insecticides
  • Need for sustainable pest control methods
  • Previous limitations in the mass production of fungal conidia

Methods Used

  • Mass production techniques for entomopathogenic fungi
  • Use of South African isolates of Metarhizium species
  • Sterile procedures for culturing and harvesting fungal conidia

Main Results

  • Successful mass production of infective conidia
  • Techniques validated under sterile conditions for optimal yield
  • Significant differences observed in conidia yield between M. robertsii and M. pinghaense

Conclusions

  • The study provides a viable method for producing entomopathogenic fungi conidia at a commercial scale.
  • The findings support the development of eco-friendly pest control solutions in agriculture.

Frequently Asked Questions

What is entomopathogenic fungi?
Entomopathogenic fungi are fungi that can cause disease in insects, making them useful for biological pest control.
Why is there a need for biological control agents?
Conventional insecticides are increasingly restricted due to their harmful effects on the environment and human health.
What are the key benefits of using entomopathogenic fungi?
They offer a more sustainable and environmentally friendly approach to managing agricultural pest populations.
How are fungal conidia produced in this study?
The study outlines a detailed method using sterile techniques and specific substrates to mass-produce fungal conidia.
What challenges does this method address?
It addresses challenges in the efficient and sterile mass production of viable fungal conidia for agricultural use.
What is the significance of the findings?
The results foster advancements in biological control practices, potentially reducing reliance on chemical pesticides in agriculture.

Entomopathogenic fungi have gained importance as the biological control agents of agricultural insect pests. In this study, the mass production of a sufficient number of resilient infective propagules of South African isolates of both Metarhizium robertsii and M. pinghaense for commercial application against insect pests was successfully conducted using agricultural grain products.

This method provides new information on how to mass produce infective conidia of entomopathogenic fungi to control important insect pests in commercial agro-ecosystems, given the current situation where most insecticides are phased out due to concerns of their toxic effect on the environment and human health. The technique describes the method used to ensure the optimum yield of EPF conidia during mass production. The method is helpful for integrated pest management and biological control by providing insight into the effectiveness of mass produced conidia for large-scale management of damaging insecticide resistance insect pests in the agro-ecosystems.

This procedure was developed and demonstrated by Dr.Letodi Luki Mathulwe, Dr.Nomakholwa Faith Stokwe, and Professor Antoinette Paula Malan. Heat one liter distilled water and switch off the heat before reaching the boiling point. Now, add 30 grams of glucose, four grams of potassium phosphate dibasic, 20 grams of yeast extract, 15 milliliters of corn steep liquor, and bring the contents to a gentle boil for two to three minutes.

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Entomopathogenic FungiMetarhizium RobertsiiMetarhizium PinghaenseMass ProductionInfective ConidiaInsect PestsIntegrated Pest ManagementBiological ControlAgro-ecosystemsFungal CultureSterile ConditionsBacterial ContaminationGlucose MediumIncubation ProcessFermentation Bag

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