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Purification of Endogenous Drosophila Transient Receptor Potential Channels
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Biochemistry
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JoVE Journal Biochemistry
Purification of Endogenous Drosophila Transient Receptor Potential Channels

Purification of Endogenous Drosophila Transient Receptor Potential Channels

DOI:
10.3791/63260-v

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08:39 min

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December 28, 2021

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, , , , ,
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute,Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center

Chapters

  • 00:04Introduction
  • 00:53Purification of GST-Tagged TRP and His-Tagged NORPA Fragment
  • 03:47Preparation of Drosophila Heads
  • 04:22Drosophila TRP Channel Purification
  • 06:15Size-Exclusion Column Purification of DrosophilaTRP Channel
  • 06:35Results: Purification of Endogenous Drosophila TRP Channel
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

Based on the assembling mechanism of the INAD protein complex, in this protocol, a modified affinity purification plus competition strategy was developed to purify the endogenous Drosophila TRP channel.

Tags

DrosophilaTransient Receptor Potential (TRP) ChannelsProtein PurificationGST-tagged TRPSize Exclusion ChromatographyE. Coli ExpressionINAD Protein ComplexHigh-pressure Homogenization

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