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Effektiv oral RNA interferens (RNAi) administration til voksne anopheles gambiae myg
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Effektiv oral RNA interferens (RNAi) administration til voksne anopheles gambiae myg

Article DOI: 10.3791/63266-v • 07:48 min • March 1st, 2022
March 1st, 2022 •
1,2, 1, 3, 4,5, 5,6, 1, 5,7,8
1Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, 2Department of Entomology, Cornell University, 3Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, 4Department of Cell Biology, Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology, Johns Hopkins School of Medicine, 8Biomedical Sciences Department, Idaho College of Osteopathic Medicine

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Den orale administration af dsRNA produceret af bakterier, en leveringsmetode til RNA-interferens (RNAi), der rutinemæssigt anvendes i Caenorhabditis elegans, blev med succes anvendt her på voksne myg. Vores metode giver mulighed for robuste reverse genetics-undersøgelser og transmissionsblokerende vektorstudier uden brug af injektion.

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Biologi udgave 181
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