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1Division of Parasitic Diseases and Malaria, Entomology Branch,Centers for Disease Control and Prevention, 2Department of Entomology,Cornell University, 3Centro de Estudios en Biotecnologia,Universidad del Valle de Guatemala, 4Department of Cell Biology,Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute,Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology,Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology,Johns Hopkins School of Medicine, 8Biomedical Sciences Department,Idaho College of Osteopathic Medicine
Chapters
- 00:05Introduction
- 00:45Preparation of Heat-Killed Bacteria Expressing Double-Stranded RNA (dsRNA)
- 02:09Feeding Mosquitoes with Heat-Killed Bacteria Expressing dsRNA
- 02:47Assay Target Gene Expression Levels
- 03:59Phenotypic Evaluation: Successful Blood-Feeding
- 04:35Phenotypic Evaluation: Salivary Gland Morphology and Down-Regulation of Relevant Proteins
- 05:30Results: The Effects of fkh Knockdown in Adult Anopheles gambiae Salivary Glands
- 07:17Conclusion
Den orala administreringen av dsRNA som produceras av bakterier, en leveransmetod för RNA-interferens (RNAi) som rutinmässigt används i Caenorhabditis elegans, tillämpades framgångsrikt här på vuxna myggor. Vår metod möjliggör robusta omvända genetikstudier och transmissionsblockerande vektorstudier utan användning av injektion.
