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1Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital,Affiliated Hospital of Fujian Medical University
Chapters
- 00:05Introduction
- 01:13Construction of GSK923295-Mediated Centromere Protein E (CENP-E) Inhibition Mouse Models
- 02:18Hematoxylin-eosin (HE) Staining and Histopathology
- 03:57Immunofluorescence and Confocal Microscopy
- 05:31Flow Cytometry
- 07:04Results: Analysis of Seminiferous Tubules and the Disruption of Spermatogenesis Post CENP-E Inhibition by GSK923295
- 08:59Conclusion
This article reports an in vivo inhibition of CENP-E through abdominal surgery and testicular injection of GSK923295, a valuable model for male meiotic division. Using the immunofluorescence, flow cytometry and transmission electron microscopy assays, we show that CENP-E inhibition results in chromosome misalignment and genome instability in mouse spermatocytes.
