3,908 Views
•
06:26 min
•
November 10, 2021
DOI:
Summary
Automatically generated
The current protocol details the isolation of phycobilisomes from cyanobacteria by centrifugation through a discontinuous sucrose density gradient. The fractions of intact phycobilisomes are confirmed by 77K fluorescent emission spectrum and SDS-PAGE analysis. The resulting phycobilisome fractions are suitable for negative staining of TEM and mass spectrometry analysis.
Related Videos
11:28
Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
Related Videos
7751 Views
08:07
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Related Videos
15246 Views
06:58
Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Related Videos
6507 Views
07:10
Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
Related Videos
1095 Views
09:43
Isolation and Characterization of RNA-Containing Exosomes
Related Videos
99421 Views
10:09
Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus
Related Videos
6245 Views
11:47
Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna
Related Videos
2672 Views
08:44
Isolation and Characterization of Cyanobacterial Extracellular Vesicles
Related Videos
5577 Views
10:35
Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria
Related Videos
2794 Views
N/A
Immunocytochemical Visualization of Proteins from Cyanobacterial Cells with High Autofluorescence of Phycoerythrin and Phycourobilin
Related Videos
222 Views
Read Article
Cite this Article
Jiang, H., Ho, M. Isolation and Characterization of Intact Phycobilisome in Cyanobacteria. J. Vis. Exp. (177), e63272, doi:10.3791/63272 (2021).
Copy