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Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
 

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Article DOI: 10.3791/63272-v
November 10th, 2021

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Summary November 10th, 2021

The current protocol details the isolation of phycobilisomes from cyanobacteria by centrifugation through a discontinuous sucrose density gradient. The fractions of intact phycobilisomes are confirmed by 77K fluorescent emission spectrum and SDS-PAGE analysis. The resulting phycobilisome fractions are suitable for negative staining of TEM and mass spectrometry analysis.

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