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Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

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06:26 min

November 10, 2021

DOI:

06:26 min
November 10, 2021

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Summary

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The current protocol details the isolation of phycobilisomes from cyanobacteria by centrifugation through a discontinuous sucrose density gradient. The fractions of intact phycobilisomes are confirmed by 77K fluorescent emission spectrum and SDS-PAGE analysis. The resulting phycobilisome fractions are suitable for negative staining of TEM and mass spectrometry analysis.

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