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JoVE Journal Bioengineering
Imaging ad autofluorescenza per valutare il metabolismo cellulare
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Imaging ad autofluorescenza per valutare il metabolismo cellulare

Article DOI: 10.3791/63282-v • 07:36 min • November 15th, 2021
November 15th, 2021 •
1, 1, 1, 1, 1
1Department of Biomedical Engineering, Texas A&M University-College Station

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Questo protocollo descrive l'imaging a fluorescenza e l'analisi dei coenzimi metabolici endogeni, della nicotinamide adenina ridotta (fosfato), del dinucleotide (NAD(P)H) e dell'adenina dinucleotide della flavina ossidata (FAD). L'imaging a autofluorescenza di NAD(P)H e FAD fornisce un metodo non distruttivo privo di etichette per valutare il metabolismo cellulare.

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Bioingegneria Numero 177 FLIM Metabolismo NAD(P)H FAD Fluorescenza Microscopia
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